Intracytoplasmic sperm injection (ICSI) is a conventional IVF procedure where fertilization of the egg is achieved through a different approach. Generally, fertilization occurs when a sperm attaches itself to the egg and penetrates through the outer layer. For some reason, the sperm may fail to penetrate the egg. In such a condition, ICSI can be done along with IVF to achieve pregnancy. During ICSI, a single sperm is picked and injected directly into the egg.

ICSI is performed by an experienced embryologist using specialized instruments. Though ICSI is a precise procedure for fertilization to occur, required cellular events need to take place.